Raquel Amanda Villamizar Gallardo
Universidad de Pamplona, Colombia
Title: Determination of Rotavirus in raw Colombian water by using fluoromagnetic detection
Biography
Biography: Raquel Amanda Villamizar Gallardo
Abstract
A fast, sensitive and selective detection method of rotavirus in raw water samples was developed. Micro magnetic particles were functionalized with monoclonal antibodies anti-Rotavirus and they were used to capture, concentrate and separate whole rotavirus particles in raw water samples from Pamplonita River, Pamplona, Colombia. Atomic force microscopy was used to prove the presence of anti-rotavirus antibodies and rotaviruses attached on the surface of the magnetic particles. In addition, RNA extraction, quantification and amplification was carried out to validate the microscopy results. The selectivity of the particles a sample containing a mix of enteric viruses was tested as potential competing viruses in the media. It was obtained that functionalized micromagnetic particles coupled to monoclonal anti-rotavirus antibodies are a rapid, selective and reliable tool for detecting up to 10 viral particle of rotavirus in 1 L in just 2 hours.